Helios: Sample Loader Daily Cleaning Procedure

SAMPLE CAPILLARY

*NOTE: Prior to beginning the procedure have the following on hand:

- Luer Adapter (PN 102183)
- Union Body (PN 101509)
- Type 1 ultrapure (18.2 ΩM) water (DIW)
- 2 mL Norm-Ject® luer slip syringes (VWR PN 89174-492)
- 5 mL conical tubes

→ Step 01:
Remove the nebulizer from the Nebulizer Adaptor port. Disconnect the Sample Capillary from the nebulizer and place the nebulizer in the nebulizer rest.

→ Step 02:
Connect the luer adapter to the union body. Connect the heater bulkhead end of the sample capillary to the union. Place the nebulizer end of the sample capillary into a clean 5 mL tube to catch any residual sample and DIW. Place the tube on a 5 mL tube rack. Fill the syringe with DIW. Inject DIW into sample capillary. When the droplets fall consistently remove the syringe and place the sample capillary in a clean, dry area.

SAMPLE LINE

→ Step 01:
Disconnect the Sample Line from the side of the Sample Loader. Disconnect the other end of the sample line from underneath the Sample Loader, remove the tan nut and place aside. Place the end into a clean 5 mL tube. Place the 5 mL tube (with the sample line end inserted) onto a 5 mL tube rack.

→ Step 02:
Connect the syringe to the luer adapter and union body. Using gentle pressure, inject DIW through the luer adapter and catch the debris and water in the 5 mL tube.

SAMPLE PROBE LINE

→ Step 01:
Connect the syringe to the luer adapter.

→ Step 02:
Open the handle of the Sample Loader and place clean 5 mL tube into the sample holder. Close the handle.

→ Step 03:
Load the syringe with DIW. Connect the syringe to the luer adapter. Carefully inject DIW through the luer adapter until a few droplets have come out of the sample probe line. Steady pressure will be required to create adequate flow into the tube. Reconnect the sample line to the side of the sample loader. Remove and discard the 5 mL tube.

GROUNDING NUT

→ Step 01:
Remove the luer adapter from the union. Connect the luer adapter to the back side of the grounding nut and connect a syringe containing DIW to the luer adapter. Gently, inject DIW through the grounding nut. Place a 50 mL conical tube on the front side of the grounding nut to catch any debris that comes out of the grounding nut. Remove and discard the 50 mL tube. Remove the luer adapter from the grounding nut.

*NOTE: Use a Kimwipe™ to dry any residual water from the grounding nut.

→ Step 02:
Reconnect the sample line to the grounding nut. Reconnect the sample capillary to the grounding nut. Reconnect the other end of the sample capillary to the sample inlet of the nebulizer. The capillary should reach the tapered portion of the sample inlet. Insert the nebulizer into the nebulizer adapter port and tighten clockwise.